Exploration of the candidate beneficial bacteria for Penaeus vannamei culture by core microbiome analysis using amplicon sequencing.

Globally, Penaeus vannamei is the vital species in aquaculture production. Beneficial bacterial exploration of gut, sediment, and water were investigated in P. vannamei culture using Illumina Miseq sequencing of 16S RNA V3-V4 hypervariable regions. Predominant phyla identified were Proteobacteria, Tenericutes, Bacteroidetes in gut; Proteobacteria, Bacteroidetes, Planctomycetes in sediment and Cyanobacteria, Proteobacteria, and Planctomycetes in water. In total, 46 phyla, 509 families and 902 genera; 70 phyla, 735 families and 1255 genera; 55 phyla, 580 families and 996 genera were observed in gut, sediment and water, respectively. Diversity of microbial communities in respect of observed Operational Taxonomic Units, diversity indices (Shannon and Simpson), richness index (Chao1) were significantly high P (<0.05) in 60 DoC in gut and 30 DoC in sediment. Beta diversity indicated separate clusters for bacterial communities in gut, sediment and water samples and formation of distinct community profiles. Core microbiome in P. vannamei rearing ponds over a time consisted of 9, 21, and 20 OTUs in gut, rearing water and sediment, respectively. This study helps to intervene with suitable beneficial microbes to establish an aquaculture system thereby contributes to enhance the productivity, improve water quality and pond bottom condition, and control the pathogenic agents at each stage of the culture.

Assessment of land and water ecosystems capability to support aquaculture expansion in climate-vulnerable regions using analytical hierarchy process based geospatial analysis.

The fast-unregulated expansion of shrimp aquaculture led to increasing ecological concerns and conflicts among the coastal resource users worldwide, thus calling for techniques to identify the suitable aquaculture zones to ensure sustainability. The multi-criteria decision-support approach was used to assess the ecosystem's ability to support the shrimp farming expansion using a hierarchical analytical process based on multiple criteria decision analysis, and the assessment of the carrying capacity of the source water bodies. Eighteen thematic layers were grouped into four major groups, namely land type, source water quality, soil characteristics, and infrastructure availability. The pairwise comparison matrix was used to assign the weights to each criterion based on its relative importance. Spatial restriction rules were framed based on the guidelines of the Coastal Aquaculture Authority of India. The favorable conditions exist in 85-100% area for expanding aquaculture in terms of water, soil, and resource availability, but the land was a major controlling factor. The extent of the appropriate area for shrimp aquaculture was found to be 7426 ha. The carrying capacity of twelve source water bodies in the study region indicates that 83.3% of water bodies can accommodate the total identified area associated with it, but the rest 17.7% water bodies can withstand the development up to70 to 72% of the available space. This approach illustrates the suitability of geospatial planning in combination with carrying capacity assessment of source water bodies for sustainable resource use in the shrimp growing nations of the world.

Effect of ammonia stress on immune variables of Pacific white shrimp Penaeus vannamei under varying levels of pH and susceptibility to white spot syndrome virus.

Of late, Pacific white shrimp Penaeus vannamei culture has intensified globally and is a major contributor to the cultured shrimp produced worldwide. Intensification of its culture has led to elevated ammonia concentration during grow-out. Ammonia toxicity is a function of water pH, temperature, salinity and beyond the optimum range, creates stress to cultured aquatic species which can reduce growth, increase susceptibility to diseases and eventually mortality. The present study was aimed at quantifying the toxic effect of total ammonia nitrogen (TAN) (1, 3, 6 & 9 mg/l) and pH levels (6, 8 & 10) individually and in combination on median survival (50% lethal time) of shrimp (8 g) after exposure for 14 days followed by post-stress challenge with white spot syndrome virus (WSSV) for 9 days. Mortality risk factor and the toxicity effect on the immune variables were evaluated. Individual stressors showed a risk factor of 1-13 times, whereas combined treatments considerably increased the risk of dying compared to control. Low survival (15%) was observed in pH6TAN9 and pH10TAN3 treatments and was substantiated by prominent histological obliteration in gills of shrimp. The cumulative mortality in post-stress WSSV challenged trials was 1-5 times and 1-35 times in individual and combination treatments, respectively compared to control. The study revealed that variations in ammonia and pH beyond the optimal range significantly influence the non-specific immune mechanisms in P.vannamei and increases the susceptibility to WSSV especially in combination treatments.

Shoreline change and potential sea level rise impacts in a climate hazardous location in southeast coast of India.

Climate change impact on the environment makes the coastal areas vulnerable and demands the evaluation of such susceptibility. Historical changes in the shoreline positions and inundation based on projected sea-level scenarios of 0.5 and 1 m were assessed for Nagapattinam District, a low-lying coastal area in the southeast coast of India, using high-resolution Shuttle Radar Topography Mission data; multi-dated Landsat satellite images of 1978, 1991, 2003, and 2015; and census data of 2011. Image processing, geographical information system, and digital shoreline analysis system methods were used in the study. The shoreline variation indicated that erosion rate varied at different time scales. The end point rate indicated the highest mean erosion of - 3.12 m/year, occurred in 73% of coast between 1978 and 1991. Weighted linear regression analysis revealed that the coast length of 83% was under erosion at a mean rate of - 2.11 m/year from 1978 to 2015. Sea level rise (SLR) impact indicated that the coastal area of about 14,122 ha from 225 villages and 31,318 ha from 272 villages would be permanently inundated for the SLR of 0.5 and 1 m, respectively, which includes agriculture, mangroves, wetlands, aquaculture, and forest lands. The loss of coastal wetlands and its associated productivity will severely threaten more than half the coastal population. Adaptation measures in people participatory mode, integrated into coastal zone management with a focus on sub-regional coastal activities, are needed to respond to the consequences of climate change.

Methodological approach for the collection and simultaneous estimation of greenhouse gases emission from aquaculture ponds.

Global warming/climate change is the greatest environmental threat of our time. Rapidly developing aquaculture sector is an anthropogenic activity, the contribution of which to global warming is little understood, and estimation of greenhouse gases (GHGs) emission from the aquaculture ponds is a key practice in predicting the impact of aquaculture on global warming. A comprehensive methodology was developed for sampling and simultaneous analysis of GHGs, carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) from the aquaculture ponds. The GHG fluxes were collected using cylindrical acrylic chamber, air pump, and tedlar bags. A cylindrical acrylic floating chamber was fabricated to collect the GHGs emanating from the surface of aquaculture ponds. The sampling methodology was standardized and in-house method validation was established by achieving linearity, accuracy, precision, and specificity. GHGs flux was found to be stable at 10 ± 2 °C of storage for 3 days. The developed methodology was used to quantify GHGs in the Pacific white shrimp Penaeus vannamei and black tiger shrimp Penaeus monodon culture ponds for a period of 4 months. The rate of emission of carbon dioxide was found to be much greater when compared to other two GHGs. Average GHGs emission in gha-1 day-1 during the culture was comparatively high in P.vannamei culture ponds.